Microinjectors are often used in genetic engineering and research, organism cloning, and in cell biology and viral studies. Clinical applications include in vitro fertilization, intracytoplasmic sperm injection, Organoids, Zebrafish, C Elegans, and Xenopus to name a few. Microinjection products are more technical and niche. Using technology that has been improved and innovated repeatedly since the 1990s, the third generation Drummond Nanoject III is incredibly accurate offering the specificity required for this type of measurement. Microinjectors are not to be used on humans.
Microinjection is the process of inserting genetic material into an animal cell or embryo using a glass micropipette/injection needle to penetrate the cell membrane. It is essentially a microscope with an incorporated micromanipulator for precise needle positioning.
Both units utilize the same glass capillaries for pulling micropipettes. Due to the new improved method of securing the micropipette, smaller tips can be pulled and utilized on the Nanoject III. No O-rings to wear out. A micro “chuck” is used, and it simplifies attachment of the micropipette once backfilled with oil. The Nanoject III’s auto HOMING feature will eliminate the need to “rehome the unit as in the Nanoject II. This can reduce, if not totally eliminate, jamming of the plunger. Now available for the Nanoject II is the new Digital Control Box which has all the functionality of the original control box but now features a user friendly, easy to read touchscreen.
|Product Name||Nanoject II||Nanoject III|
|Injection volumes||2.3 nL-69 nL||0.6 nL-999.9 nL|
|Rate of injection||46 nL/sec.& 23 nL/sec. only||1 nL/sec.-200 nL/sec.|
|Programmable multiple injections||No||Yes|
|Programmable frequency between injections||No||Yes|
|Auto HOMING of plunger||No||Yes|
|Micropipette I.D. size||10 µm minimum||2-3 µm minimum|
|Optional Footswitch||Yes; inject only||Yes; fill, empty and inject capability|
|Color, Touch Sensitive Keypad||No; fill, empty, inject buttons only. Optional Digital Control Box Upgrade available||Yes; multi screens|
|External computer interface||Use of footswitch contact to inject. Injector has to be finished its injection at the speed rate of the Nanoject II before it will accept another pulse||Use of a special opto-isolated input to allow the user to control injection rate and injection volume|
Use this comparison chart to understand the difference between Drummond Pipet-Aid® Controller models and their useful features. This information will help you choose the best pipette controller to deliver the most accurate results for your intended applications.
|Feature||Other Pipette Controllers||Portable Pipet- Aid® XP Pipette Controller||Portable Pipet- Aid® XL Pipette Controller||Pipet- Aid® Hood Mate® Pipette Controller|
|3 Speed Aspiration & Dispensing||X||✓||✓||✓|
|Use in hood/laminar flow cabinet||X||X||✓||✓|